Organelles are the defining characteristic of eukaryotic cells. The membranes that enclose organelles are tightly regulated in response to stimuli and stress. One way that lipids are regulated is through the saturation state of their acyl chains. This aspect of the membrane environment has an enormous effect on how membrane proteins insert into membranes, form complexes and interactions, and function. This research investigates the cellular consequences of dysregulated lipid saturation, and the pathways required to adapt to this type of membrane stress. These fundamental studies has broad implications in multiple diseases across several cell types. Our work uses budding yeast as a model, and techniques in genetics, microscopy, molecular biology, and biochemistry.